LILRA5/CD85f in Human PBMCs. LILRA5/CD85f was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human LILRA5/CD85f Monoclonal Antibody (Catalog # MAB6754) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007 ) and counterstained with DAPI (blue). Specific staining was localized to surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells .
Detection of LILRA5/CD85f in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human LILRA5/CD85f Monoclonal Antibody (Catalog # MAB6754, filled histogram) or isotype control antibody (Catalog # MAB003 , open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B ).