All lanes : Anti-C21orf59 antibody [EPR13320-66] (ab181173) at 1/5000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%), labeling C21orf59 with ab181173 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot analysis of Jurkat cell lysate immunoprecipitated using ab181173 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution.