All lanes : Anti-C2orf40 antibody [1A10] (ab119079) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY C2orf40 Lysates/proteins at 5 µg per lane.
HEK293T cells transfected with either C2orf40 (Red) or empty vector control (Blue) were immunostained by ab119079 at a dilution of 1/100, then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using ab119079 at a dilution of 1/100 (Red), compared to a nonspecific negative control antibody (Blue).