NETO1 in MOLT‑4 Human Cell Line. NETO1 was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010 ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells .
NETO1 in Human Prostate. NETO1 was detected in immersion fixed paraffin-embedded sections of human prostate using Sheep Anti-Human NETO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4337) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019 ) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .