Detection of Human and Mouse TAFA5/FAM19A5 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line, T98G human glioblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat TAFA5/ FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TAFA5/FAM19A5 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Detection of TAFA5/FAM19A5 in IMR-32 Human Cell Line by Flow Cytometry. IMR-32 human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat TAFA5/ FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148, filled histogram) or control antibody (Catalog # AB-108-C , open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108 ).