Anti-C3c antibody (FITC)
| Name | Anti-C3c antibody (FITC) |
|---|---|
| Supplier | Abcam |
| Catalog | ab4212 |
| Prices | $378.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Sheep, Goat, Guinea Pig, Bovine, Cat, Dog, Human, Pig, Kangaroo, Mink |
| Antigen | C3c complement isolated from complement activated human serum |
| Description | Rabbit Polyclonal |
| Gene | C3 |
| Conjugate | FITC |
| Supplier Page | Shop |
Product images
ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.