Detection of Human FABP8/M-FABP by Western Blot Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human FABP8/M-FABP Monoclonal Antibody (Catalog # MAB5866) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for FABP8/M-FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
FABP8/M-FABP in Human Spinal Cord. FABP8/M-FABP was detected in immersion fixed paraffin-embedded sections of human spinal cord using using Mouse Anti-Human FABP8/ M-FABP Monoclonal Antibody (Catalog # MAB5866) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013 ). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002 ) and counterstained with hematoxylin (blue). Specific staining was localized to white matter. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections .