Detection of Human, Mouse, and Rat Follistatin-like 4/FSTL4 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019 ). A specific band was detected for Follistatin-like 4/FSTL4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Follistatin-like 4/FSTL4 in Mouse Brain. Follistatin-like 4/FSTL4 was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008 ) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons in the brainstem. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections . This application has not been tested in human or rat samples