Detection of Human, Mouse, and Rat Follistatin‑like 4/FSTL4 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue, rat brain (cortex) tissue, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Follistatin-like 4/FSTL4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Follistatin-like 4/FSTL4 in Mouse Brain. Follistatin-like 4/FSTL4 was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Human/Mouse/Rat Follistatin-like 4/FSTL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons in the brainstem. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. This application has not been tested in human or rat samples