Anti-C4 binding protein antibody
| Name | Anti-C4 binding protein antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab83755 |
| Prices | $378.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P |
| Species Reactivities | Human |
| Antigen | Synthetic peptide corresponding to a region within internal sequence amino acids 548-597 (QVLTGKRLMQ CLPNPEDVKM ALEVYKLSLE IEQLELQRDS ARQSTLDKEL) of human C4 binding protein alpha chain precursor Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | C4BPB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-C4 binding protein antibody (ab83755) at 1 µg/ml + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
IHC image of ab83755 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab83755, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.