Detection of Mouse Amnionless by Western Blot. Western blot shows recombinant mouse Amnionless (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Amnionless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7139) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Amnionless at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
Amnionless in Mouse Kidney. Amnionless was detected in perfusion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Amnionless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7139) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010 ) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections .