Detection of Claudin‑17 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Claudin‑17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab') 2 Secondary Antibody (Catalog # F0101B ). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Claudin‑17 in HEK293 Human Cell Line Transfected with Human Claudin-17 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Claudin-17 was stained with Mouse Anti-Human Claudin‑17 Monoclonal Antibody (Catalog # MAB4619, filled histogram) or isotype control antibody (Catalog # MAB0041 , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B ). View our protocol for Staining Membrane-associated Proteins .