Detection of Bovine SPAM1 by Western Blot. Western blot shows lysates of recombinant bovine SPAM1 (Catalog # 6436-GH ). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Bovine SPAM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6436) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for SPAM1 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .