Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121406 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121406 at 1/20 dilution staining C7orf53 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
All lanes : Anti-C7orf53 antibody (ab121406) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : C7orf53-transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))developed using the ECL technique