All lanes : Anti-C8orf30A antibody [EPR13203-28] (ab181031) at 1/5000 dilutionLane 1 : Human fetal liver lysateLane 2 : Human spleen lysateLane 3 : HeLa lysateLane 4 : Caco2 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrium tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (+) or a negative control (-) immunoprecipitated using ab181031 at 1/40 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling C8orf30A with ab181031 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.