LCoR in MCF-7 Human Cell Line. LCoR was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human LCoR Monoclonal Antibody (Catalog # MAB7534) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007 ) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .