Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli, nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121659 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lane 1 : Lanes 2 - 3 : Anti-C9orf135 antibody (ab121659)Lane 1 : MarkerLane 2 : HEK293T cell lysate - vector only transfectedLane 3 : HEK293T cell lysate - overexpressing C9orf135 with a C-terminal myc-DDK tag (~3.1kDa)
ab121659, at 1/500 dilution, staining C9orf135 in paraffin-embedded Human stomach tissue by Immunohistochemistry.