Detection of Human Olfactomedin-2/Noelin-2 by Western Blot. Western blot shows lysates of human cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Olfactomedin-2/Noelin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Olfactomedin-2/ Noelin-2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .