All lanes : Anti-C9orf40 antibody (ab202286) at 0.1 µg/mlLane 1 : Whole cell lysate from HeLa cellsLane 2 : Whole cell lysate from 293T cellsLane 3 : Whole cell lysate from Jurkat cellsLane 4 : Whole cell lysate from mouse TCMK-1 cellsLane 5 : Whole cell lysate from mouse NIH 3T3 cellsLysates/proteins at 50 µg per lane.developed using the ECL technique
Lane 1: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognizes an epitope of C9orf40.Lane 2: Detection of Human C9orf40 by Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202286 at 6 µg per reaction for IP.Lane 3: Immunoprecipitation from 293T whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.Subsequent Western blot detection used ab202286 at 0.4 µg/ml. Detection: Chemiluminescence with an exposure timeof 3 minutes.