Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121527 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121527 at 1/500 dilution staining C9ORF91 in Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
All lanes : Anti-C9ORF91 antibody (ab121527) at 1/250 dilutionLane 1 : Vector only transfected HEK293T cell lysateLane 2 : C9ORF91 over-expression HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)developed using the ECL technique