Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab195043 at 1/30 dilution (lane 1) or a Rabbit monoclonal IgG (lane 2).Western Blot performed using ab195043 at 1/1000 dilution.Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Flow cytometric analysis of U87-MG cells (paraformaldehyde-fixed, 2%) labeling CA6 with ab195043 at 1/20 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
Anti-CA6 antibody [EPR13953(2)] (ab195043) at 1/2000 dilution + Human fetal brain lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-CA6 antibody [EPR13953(2)] (ab195043) at 1/1000 dilutionLane 1 : THP1 cell lysateLane 2 : U87-MG cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique
Anti-CA6 antibody [EPR13953(2)] (ab195043) at 1/1000 dilution + 293 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilutiondeveloped using the ECL technique