All lanes : Anti-CA7 [EPR13304] antibody (ab191408) at 1/5000 dilutionLane 1 : Human fetal brain lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-CA7 [EPR13304] antibody (ab191408) at 1/20000 dilution + Human fetal kidney lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-CA7 [EPR13304] antibody (ab191408) at 1/5000 dilution + Raw264.7 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescence analysis of HeLa cells labeling CA7 using ab191408 at 1/50 dilution (4 μg/ml). A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde and permealized with 0.1% triton X-100. Counterstain: DAPI. The 2 negative cotrol images were obtained using Goat anti Mouse IgG (Alexa Fluor®594) at 1:400 dilution as secondary antibody.
Immunoprecipitation analysis of Human fetal brain tissue lysate labeling CA7 using ab191408 at 1/20 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Human fetal brain tissue lysate.