All lanes : Anti-CACNA2D2 antibody [EPR12343-17] (ab181201) at 1/10000 dilutionLane 1 : Human cerebellum lysateLane 2 : Human fetal brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-CACNA2D2 antibody [EPR12343-17] (ab181201) at 1/2000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CACNA2D2 with ab181201 at 1/100 dilution. The slide is counterstained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling CACNA2D2 with ab181201 at 1/100 dilution. The slide is counterstained with Hematoxylin.
Immunofluorescence analysis of -20°C acetone-fixed HeLa cells labeling CACNA2D2 with ab181201 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).
Immunoprecipitation of Human fetal brain lysates using ab181201. Detection of CACNA2D2 utilised ab181201 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.