Detection of Mouse GFRa-like by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse GFRa-like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). Specific bands were detected for GFRa-like at approximately 26 and 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .
GFRa-like in Mouse Brain. GFRa-like was detected in immersion fixed frozen sections of mouse brain using Sheep Anti-Mouse GFRa-like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010 ) and counterstained with Fluoro Nissl Green (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections .