Anti-CACNB3 antibody - C-terminal
| Name | Anti-CACNB3 antibody - C-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab79264 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide from the C terminal region of Rat CACNB3 conjugated to an immunogenic carrier protein |
| Description | Rabbit Polyclonal |
| Gene | CACNB3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-CACNB3 antibody - C-terminal (ab79264)Lane 1 : Lystates prepared from mouse hippocampal homogenate tissue preparation.Lane 2 : Lystates prepared from mouse hippocampal synaptosome tissue preparation.Lane 3 : Lystates prepared from large synaptosomes from the hippocampal mossy fiber pathway.Lane 4 : Lystates prepared from further resolved large synaptosomes from the hippocampal mossy fiber pathway with medullated fibers removed.Lysates/proteins at 5 µg per lane.SecondaryHRP-conjugated Goat polyclonal to rabbit IgG at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![CACNB3 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CACNB3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79264.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 66kDa, non specific band - 45kDa: We are unsure as to the identity of this extra band; CACNB3](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19049_CACNB3-Primary-antibodies-ab79264-3.jpg)
CACNB3 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CACNB3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab79264.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 66kDa, non specific band - 45kDa: We are unsure as to the identity of this extra band; CACNB3
Product References
Identification of new dystroglycan complexes in skeletal muscle. - Identification of new dystroglycan complexes in skeletal muscle.
Johnson EK, Li B, Yoon JH, Flanigan KM, Martin PT, Ervasti J, Montanaro F. PLoS One. 2013 Aug 8;8(8):e73224.
The beta3 subunit contributes to vascular calcium channel upregulation and - The beta3 subunit contributes to vascular calcium channel upregulation and
Kharade SV, Sonkusare SK, Srivastava AK, Thakali KM, Fletcher TW, Rhee SW, Rusch NJ. Hypertension. 2013 Jan;61(1):137-42.