Detection of Mouse Intelectin‑2 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Intelectin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5358) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for Intelectin-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8 .