Anti-CAD antibody [EP710Y]
| Name | Anti-CAD antibody [EP710Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab40800 |
| Prices | $357.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP710Y |
| Applications | ICC/IF ICC/IF WB FC IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in the N-terminus of human CAD |
| Description | Rabbit Monoclonal |
| Gene | CAD |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-CAD antibody [EP710Y] (ab40800) at 1/1000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19099_ab40800_WB_1.jpg)
Anti-CAD antibody [EP710Y] (ab40800) at 1/1000 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902) at 10 µg
Overlay histogram showing HeLa cells stained with ab40800 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40800, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.