Podocan in Mouse Kidney. Podocan was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse Podocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3104) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008 ) and counterstained with hematoxylin (blue). Specific labeling was localized to vascular endothelial cells within glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections .