SEZ6L in Mouse Brain. SEZ6L was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Mouse SEZ6L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4804) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010 ) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections .