Detection of Mouse ZIP4H/Tex11 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line non-transfected or transfected with recombinant mouse ZIP4H or recombinant human ZIP4H. PVDF membrane was probed with 0.5 µg/mL Rat Anti-Mouse ZIP4H/Tex11 Monoclonal Antibody (Catalog # MAB5627) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). A specific band for ZIP4H/Tex11 was detected at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .