Anti-Calcineurin A antibody
| Name | Anti-Calcineurin A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3673 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Full length Calcineurin A fusion expressed in E |
| Description | Rabbit Polyclonal |
| Gene | PPP3CA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western Blot of wild type mouse brain homogenate with ab3673 used at 1/6000. Please note that this image was assembled from two different blots. (The numbers on the scale correspond to kD).
All lanes : Anti-Calcineurin A antibody (ab3673) at 1/200 dilutionLane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Brain (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 62 kDa (why is the actual band size different from the predicted?)
Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Lysate prepared from human HUH-7 cells at 15000 cellsSecondaryHRP conjugated sheep polyclonal to rabbit IgG at 1/15000 dilutionPerformed under reducing conditions.Observed band size : 62 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minuteThis image is a courtesy of Anonymous AbreviewSee Abreview
Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Human Calcineurin A full length protein (ab101870) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds
Immunofluorescence analysis of rat dissociated hippocampal cells, staining Calcineurin A with ab3673. Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 10% normal goat serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 2% normal goat serum) for 1 hour at 23°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody. See Abreview
Product References
Interaction between maternal and postnatal high fat diet leads to a greater risk - Interaction between maternal and postnatal high fat diet leads to a greater risk
Turdi S, Ge W, Hu N, Bradley KM, Wang X, Ren J. J Mol Cell Cardiol. 2013 Feb;55:117-29.
Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression - Hypertrophic cardiomyopathy in high-fat diet-induced obesity: role of suppression
Fang CX, Dong F, Thomas DP, Ma H, He L, Ren J. Am J Physiol Heart Circ Physiol. 2008 Sep;295(3):H1206-H1215. doi:
The Down syndrome critical region protein RCAN1 regulates long-term potentiation - The Down syndrome critical region protein RCAN1 regulates long-term potentiation
Hoeffer CA, Dey A, Sachan N, Wong H, Patterson RJ, Shelton JM, Richardson JA, Klann E, Rothermel BA. J Neurosci. 2007 Nov 28;27(48):13161-72.