Anti-Calcineurin A antibody [EP1669Y]
| Name | Anti-Calcineurin A antibody [EP1669Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52761 |
| Prices | $388.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1669Y |
| Applications | WB IP FC |
| Species Reactivities | Mouse, Rat, Hamster, Human |
| Antigen | A synthetic peptide corresponding to residues on the N terminus of human Calcineurin A |
| Description | Rabbit Monoclonal |
| Gene | PPP3CA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19214_ab52761.jpg)
Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution
Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.