Anti-Calcineurin A antibody [EPR1670(2)]

• Supplier: Abcam
• Catalog: ab109412
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1670(2)
• Applications: WB FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues near the C-terminus of Human Calcineurin A
• Description: Rabbit Monoclonal
• Gene: PPP3CA
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab109412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109412, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilutionLane 1 : fetal brain lysateLane 2 : SH-SY5Y lysateLane 3 : A431 lysateLane 4 : HeLa cells lysateLysates/proteins at 10 µg per lane.

Immunofluorescent staining of HeLa cells using 1/100 ab109412.