Detection of Human, Mouse, and Rat UFM1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human UFM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8237) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016 ). A specific band was detected for UFM1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .
UFM1 in HeLa Human Cell Line. UFM1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human UFM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8237) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010 ) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips .