Detection of Human Transcobalamin II by Western Blot.Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Transcobalamin II Monoclonal Antibody (Catalog # MAB7895) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Transcobalamin II at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.