Anti-Calcium Pump PMCA3 ATPase antibody
| Name | Anti-Calcium Pump PMCA3 ATPase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3530 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IP WB ELISA IHC-P |
| Species Reactivities | Mouse, Rat, Human, Pig, Primate |
| Antigen | Synthetic peptide corresponding to Rat Calcium Pump PMCA3 ATPase aa 5-22 |
| Description | Rabbit Polyclonal |
| Gene | ATP2B3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab3530 at 1/200 staining rat hippocampal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubating with the antibody for 12 hours. An Alexa Fluor ®488 conjugated goat anti rabbit antibody was used secondary.See Abreview
All lanes : Anti-Calcium Pump PMCA3 ATPase antibody (ab3530) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from rat primary cortical neuronsLane 2 : Whole cell lysate prepared from rat primary cortical neuronsLane 3 : Whole cell lysate prepared from rat primary cortical neuronsLane 4 : Whole cell lysate prepared from rat primary cortical neuronsLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated secondary at 1/20000 dilutiondeveloped using the ECL technique
Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Product References
Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: - Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A:
Kosiorek M, Podszywalow-Bartnicka P, Zylinska L, Zablocki K, Pikula S. Biochem Biophys Res Commun. 2011 Jul 29;411(2):235-40. doi:
Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic - Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic
Jensen TP, Filoteo AG, Knopfel T, Empson RM. J Physiol. 2007 Feb 15;579(Pt 1):85-99. Epub 2006 Dec 14.