Detection of Human Cytosolic Sulfotransferase 1C2/SULT1C2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 1C2/SULT1C2 Monoclonal Antibody (Catalog # MAB7458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1C2/SULT1C2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytosolic Sulfotransferase 1C2/SULT1C2 in Human Stomach. Cytosolic Sulfotransferase 1C2/SULT1C2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Cytosolic Sulfotransferase 1C2/SULT1C2 Monoclonal Antibody (Catalog # MAB7458) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.