Anti-Calmegin antibody [EPR11832-11]

• Supplier: Abcam
• Catalog: ab172477
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR11832-11
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human, Chimpanzee, Marmoset
• Antigen: Recombinant fragment within Human Calmegin
• Description: Rabbit Monoclonal
• Gene: CLGN
• Conjugate: Unconjugated

Product images

All lanes : Anti-Calmegin antibody [EPR11832-11] (ab172477) at 1/10000 dilutionLane 1 : Human testis tissue lysateLane 2 : Jurkat cell lysateLane 3 : Mouse testis tissue lysateLane 4 : Rat testis tissue lysateLysates/proteins at 10 µg per lane.

IHC-P image of Calmegin staining with ab172477 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab172477 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.See Abreview

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Calmegin with ab172477 at 1/250 dilution.

Immunofluorescent analysis of Jurkat cells labeling Calmegin with ab172477 at 1/100 dilution.

Flow cytometric analysis of permeabilized Jurkat cells labeling Calmegin with ab172477 at 1/10 dilution (red) compared with a rabbit IgG (negative) (green).

Western blot analysis on immunoprecipitation pellet from Human testis lysate labeling Calmegin with ab172477 at 1/10 dilution.