Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122665 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab122665 at 1/2500 dilution staining CALML5 in paraffin-embedded Human skin tissue by Immunohistochemistry.
All lanes : Anti-CALML5 antibody (ab122665) at 1/250 dilutionLane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : CALML5 over-expression lysate; co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cellsdeveloped using the ECL technique