Anti-Calmodulin antibody [EP799Y]
| Name | Anti-Calmodulin antibody [EP799Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab45689 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP799Y |
| Applications | WB IHC-P ICC/IF ICC/IF FC IP IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal) |
| Description | Rabbit Monoclonal |
| Gene | CALM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab45689 staining Calmodulin in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% Serum for 60 minutes at 4°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% Triton) for 12 hours at 4°C. A Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.See Abreview
![Anti-Calmodulin antibody [EP799Y] (ab45689) at 1/5000 dilution + NIH 3T3 cell lysate (10ug/lane)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19526_ab45689_1.jpg)
Anti-Calmodulin antibody [EP799Y] (ab45689) at 1/5000 dilution + NIH 3T3 cell lysate (10ug/lane)
Ab45689 (1:250) staining human Calmodulin in human urinary bladder carcinoma by immunohistochemistry in paraffin embedded tissue.
ICC/IF image of ab45689 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45689, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing MCF-7 cells stained with ab45689 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45689, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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Calcium-dependent association of calmodulin with the rubella virus nonstructural - Calcium-dependent association of calmodulin with the rubella virus nonstructural
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