Anti-Calnexin antibody - ER Marker
| Name | Anti-Calnexin antibody - ER Marker |
|---|---|
| Supplier | Abcam |
| Catalog | ab10286 |
| Prices | $392.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Dog, Human, Pig, Hamster |
| Antigen | His-Tag E |
| Description | Rabbit Polyclonal |
| Gene | CANX |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Calnexin antibody - ER Marker (ab10286) at 1/1000 dilution + MCF7 cellsSecondaryGoat anti rabbit at 1/10000 dilution
All lanes : Anti-Calnexin antibody - ER Marker (ab10286) at 1/4000 dilutionLane 1 : HeLa (Total cell lysate)Lane 2 : HeLa (Membrane fraction)Lane 3 : HeLa (Cytoplasmic fraction)Lane 4 : MDA-MB231 (Total cell lysate)Lane 5 : MDA-MB231 (Membrane fraction)Lane 6 : MDA-MB231 (Cytoplasmic fraction)SecondaryHRP conjugated donkey anti-rabbitdeveloped using the ECL techniquePerformed under reducing conditions.
Paraformaldehyde fixed HeLa cells stained with ab10286. For further details please refer to the customer Abreview.
ab10286 staining Calnexin in Pig retinal pigment epithelial cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 0.1% TX100; 1% goat serum; 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody.See Abreview
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