Anti-Calpain 1 antibody

• Supplier: Abcam
• Catalog: ab28258
• Prices: $395.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide based on Domain I of the large subunit of human calpain 1
• Description: Rabbit Polyclonal
• Gene: CAPN1
• Conjugate: Unconjugated

Product images

Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution + Calpain 1 at 10 µg

ab28258 staining human HUVEC cells by ICC/IF.  Cells were PFA fixed and permeablized in 0.5% Triton X-100 prior to blocking in 1% serum for 16 hours at 4°C.  The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 25°C.  A FITC conjugated goat anti-rabbit antibody, diluted 1/100, was used as the secondary.See Abreview

ab28258 detecting Calpain 1 - Domain I in Human platelets by Flow Cytometry. Platelets were isolated by spinning Platelet rich plasma on Histopaque. Cells were fixed in PFA and permeabilized in 0.1% Triton-X100 in 2% BSA for 30 minutes. The primary antibody was diluted 1/250 in 2% BSA in PBS and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Chicken anti-Rabbit IgG (H+L) antibody, diluted 1/500 was used as the secondary antibody. Abbreviations in the figure: P: Permeabilized; US Unstained, Red Peak; IgG Rb: IgG Rabbit (Isotype Control), Blue Peak; Calpain: Green Peak.See Abreview

Product References

Endoplasmic reticulum stress-associated cone photoreceptor degeneration in cyclic - Endoplasmic reticulum stress-associated cone photoreceptor degeneration in cyclic

Thapa A, Morris L, Xu J, Ma H, Michalakis S, Biel M, Ding XQ. J Biol Chem. 2012 May 25;287(22):18018-29.

Fluid shear stress and sphingosine 1-phosphate activate calpain to promote - Fluid shear stress and sphingosine 1-phosphate activate calpain to promote

Kang H, Kwak HI, Kaunas R, Bayless KJ. J Biol Chem. 2011 Dec 9;286(49):42017-26.