Flow cytometric analysis of permeabilized 293T cells labeling Calpain 15 with ab170926 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Anti-Calpain 15 antibody [EPR11078] (ab170926) at 1/1000 dilution + SH SY5Y cell lysate at 10 µgSecondaryGoat anti-rabbit HRP conjugate at 1/2000 dilution
All lanes : Anti-Calpain 15 antibody [EPR11078] (ab170926) at 1/1000 dilutionLane 1 : Human fetal lung lysateLane 2 : Human fetal brain lysateLane 3 : HeLa cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugate at 1/2000 dilution