All lanes : Anti-Calpain 9 antibody [2G1] (ab119302) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Calpain 9 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-Calpain 9 antibody [2G1] (ab119302) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab119302, at 1/100 dilution, staining Calpain 9 in COS7 cells transiently transfected with pCMV6-ENTRY Calpain 9 by Immunofluorescence.
ab119302, at 1/100 dilution, staining Calpain 9 (red) in Jurkat cells, compared to a nonspecific negative control antibody (blue) by flow cytometry.
ab119302, at 1/150 dilution, staining Calpain 9 in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
ab119302, at 1/150 dilution, staining Calpain 9 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.