Anti-Calpain S1 antibody [EPR3324]
| Name | Anti-Calpain S1 antibody [EPR3324] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92333 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3324 |
| Applications | WB IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human, Hamster |
| Antigen | Synthetic peptide (Human) |
| Description | Rabbit Monoclonal |
| Gene | CAPNS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-Calpain S1 antibody [EPR3324] (ab92333) at 1/2000 dilutionLane 1 : T47D cell lysateLane 2 : fetal brain lysateLane 3 : fetal spleen lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/19741_Calpain-S1-Primary-antibodies-ab92333-1.jpg)
All lanes : Anti-Calpain S1 antibody [EPR3324] (ab92333) at 1/2000 dilutionLane 1 : T47D cell lysateLane 2 : fetal brain lysateLane 3 : fetal spleen lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution
Immunohistochemistry staining of Calpain S1 in formalin-fixed, paraffin-embedded Human kidney tissue using 1/100 ab92333.
Overlay histogram showing HeLa cells stained with ab92333 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92333, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.