Anti-Calpastatin antibody
| Name | Anti-Calpastatin antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab28252 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide to the amino terminal end of the testis isoform of human calpstatin |
| Description | Rabbit Polyclonal |
| Gene | CAST |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab28252 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28252, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Calpastatin antibody (ab28252) at 1/5000 dilutionLane 1 : Mouse skeletal tissue muscle lysateLane 2 : Mouse skeletal tissue muscle lysateLane 3 : Mouse skeletal tissue muscle lysateLysates/proteins at 30 µg per lane.SecondaryHRP-conjugated Goat IgG to Rabbitdeveloped using the ECL techniquePerformed under reducing conditions.