Anti-Calreticulin antibody [EPR3924] - ER Marker

• Supplier: Abcam
• Catalog: ab92516
• Prices: $388.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3924
• Applications: WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human, Monkey
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: CALR
• Conjugate: Unconjugated

Product images

All lanes : Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516) at 1/1000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : HL-60 cell lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLane 5 : Fetal kidney lysateLane 6 : Fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

ab92516, at 1/250 dilution, staining Calreticulin in paraffin embedded Human kidney tissue by Immunohistochemistry.

ab92516, at 1/100 dilution, staining Calreticulin in HeLa cells by Immunofluorescence.

ab92516 showing positive staining in Normal colon tissue.

ab92516 showing negative staining in Normal heart tissue.

ab92516 showing positive staining in Normal liver tissue.

ab92516 showing positive staining in Normal placenta tissue.

ab92516 showing positive staining in Normal stomach tissue.

ab92516 showing positive staining in Papillary carcinoma of thyroid gland tissue.

Overlay histogram showing HeLa cells stained with ab92516 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92516, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

The KUPKB: a novel Web application to access multiomics data on kidney disease. - The KUPKB: a novel Web application to access multiomics data on kidney disease.

Klein J, Jupp S, Moulos P, Fernandez M, Buffin-Meyer B, Casemayou A, Chaaya R, Charonis A, Bascands JL, Stevens R, Schanstra JP. FASEB J. 2012 May;26(5):2145-53.