Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilution + Human skeletal muscle lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Calsequestrin 1 antibody [EPR15226(B)] (ab185220) at 1/10000 dilutionLane 1 : Rat muscle lysateLane 2 : Mouse muscle lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain: Hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 1 with ab185220 at 1/500 dilution. Counterstain: Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling Calsequestrin 1 with ab185220 at 1/240 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.