Anti-Calumenin antibody [EPR9075]

• Supplier: Abcam
• Catalog: ab137019
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9075
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues in Human Calumenin
• Description: Rabbit Monoclonal
• Gene: CALU
• Conjugate: Unconjugated

Product images

Overlay histogram showing JEG3 cells stained with ab137019 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137019, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

All lanes : Anti-Calumenin antibody [EPR9075] (ab137019) at 1/1000 dilutionLane 1 : Human placenta lysateLane 2 : Jurkat lysateLane 3 : 293T lysateLane 4 : K562 lysateLane 5 : HACAT lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human placanta tissue labelling Calumenin with ab137019 at 1/100 dilution.

Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional carcinoma tissue labelling Calumenin with ab137019 at 1/100 dilution.