Anti-CaMKII antibody [EP1829Y]

• Supplier: Abcam
• Catalog: ab52476
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1829Y
• Applications: ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CaMKII aa 200-300
• Blocking Peptide: CaMKII peptide
• Description: Rabbit Monoclonal
• Gene: CAMK2A
• Conjugate: Unconjugated

Product images

Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µgSecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/1000 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/20000 dilution (unpurified) + Mouse brain lysate at 10 µgSecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CaMKII with purified ab52476 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling CaMKII with purified ab52476 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% aTriton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

ICC/IF image of unpurified ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52476, 1µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Neuronally-expressed Sarm1 regulates expression of inflammatory and antiviral - Neuronally-expressed Sarm1 regulates expression of inflammatory and antiviral

Lin CW, Liu HY, Chen CY, Hsueh YP. Innate Immun. 2014 Feb;20(2):161-72.

Decreased anxiety-like behavior and Galphaq/11-dependent responses in the - Decreased anxiety-like behavior and Galphaq/11-dependent responses in the

Riccio A, Li Y, Tsvetkov E, Gapon S, Yao GL, Smith KS, Engin E, Rudolph U, Bolshakov VY, Clapham DE. J Neurosci. 2014 Mar 5;34(10):3653-67.

Transgenic mouse models enabling photolabeling of individual neurons in vivo. - Transgenic mouse models enabling photolabeling of individual neurons in vivo.

Peter M, Bathellier B, Fontinha B, Pliota P, Haubensak W, Rumpel S. PLoS One. 2013 Apr 23;8(4):e62132.

Developmental molecular and functional cerebellar alterations induced by - Developmental molecular and functional cerebellar alterations induced by

Mouton-Liger F, Sahun I, Collin T, Lopes Pereira P, Masini D, Thomas S, Paly E, Luilier S, Meme S, Jouhault Q, Bennai S, Beloeil JC, Bizot JC, Herault Y, Dierssen M, Creau N. Neurobiol Dis. 2014 Mar;63:92-106.

Ca(2+)-stimulated adenylyl cyclases regulate the L-type Ca(2+) current in - Ca(2+)-stimulated adenylyl cyclases regulate the L-type Ca(2+) current in

Collins TP, Terrar DA. J Physiol. 2012 Apr 15;590(Pt 8):1881-93.

Molecular interaction and functional regulation of ClC-3 by - Molecular interaction and functional regulation of ClC-3 by

Cuddapah VA, Sontheimer H. J Biol Chem. 2010 Apr 9;285(15):11188-96.

Essential role for TRPC5 in amygdala function and fear-related behavior. - Essential role for TRPC5 in amygdala function and fear-related behavior.

Riccio A, Li Y, Moon J, Kim KS, Smith KS, Rudolph U, Gapon S, Yao GL, Tsvetkov E, Rodig SJ, Van't Veer A, Meloni EG, Carlezon WA Jr, Bolshakov VY, Clapham DE. Cell. 2009 May 15;137(4):761-72.

Driving fast-spiking cells induces gamma rhythm and controls sensory responses. - Driving fast-spiking cells induces gamma rhythm and controls sensory responses.

Cardin JA, Carlen M, Meletis K, Knoblich U, Zhang F, Deisseroth K, Tsai LH, Moore CI. Nature. 2009 Jun 4;459(7247):663-7.